µ-Slide ibiPore Transwell细胞侵袭－85016 85026 85036 85046

订购信息：

参数：

Adapters	Female Luer or  ibidi Adapter for  20/200 µl pipet tips
Volume of main channel	50 µl
Height of main channel	0.4 mm
Length of main channel	25 mm
Width of main channel	5 mm
Growth area, main channel	1.25 cm²
Volume of upper channel	30 µl
Height of upper channel	various
Bottom: ibidi Polymer Coverslip

 

Specifications ibiPore Membrane:

Material	SiO2 (glass)
Thickness	0.3 µm (300 nm)
Membrane size	2 mm x 2 mm
Available variations	Pore Size 0.5 µm 3 µm 5 µm 8 µm Porosity 20% 5% 5% 5%

应用:

1.流动状态下跨内皮细胞迁移

2.2D或者3D凝胶内细胞层共培养和运输

3.顶部-基底细胞急性分析

4.液体-空气接触的皮肤和肺部模型

5.顶部-基底梯度的细胞屏障模型

6.基于过滤系统和孔状膜的细胞迁移分析

技术特点:

1.SiMPore’s G-FLAT™ 微孔径玻璃膜

2.中间具有玻璃光学膜的跨通道结构

3.与盖玻片相比，具有较好的光学特质

4.孔径大小0.5μm，3μm，5μm，8μm

5.膜厚度0.3μm（300nm）

6.物镜工作距离>0.5mm

7.完全兼容ibidi泵系统

8.特定剪切力大小和剪切力速率水平
(详情点击： Application Note 11)

Endothelial cells on 3 µm ibiPore membrane. Immunofluorescence overlay image of phase contrast, DAPI (blue), VE-Cadherins (green), F-Actin (red).

Fibroblast cells on 3 µm ibiPore membrane. 
Phase contrast image, objective lens 4x.

 

应用建议: 孔径 & 孔密度

膜类型	应用	细胞种类案例
0.5 µm pores,  high porosity (20%)	Permeabilitytransport studies,  co-culture models.  With this model, cell migration is not possible.	Lung cells, epithelial cells
3.0 µm pores,  low porosity (5%)	Transendothelial migration	Leukocytes (neutrophils, T-cells)
5.0 µm pores,  low porosity (5%)	Invasion, migration	Monocytes, macrophages, lymphocytes
8.0 µm pores,  low porosity (5%)	Invasion, migration	Tumorcancer cells, endothelialepithelial cells, fibroblasts, osteoblasts, melanoma, glioma

越大的细胞 – 建议使用越大的孔径。 

不同应用的建议孔径：

µ-Slide ibiPore

µ-Slide ibiPore 细胞侵袭载玻片结构

交叉通道结构和中间ibiPore膜结构的3D示意图

应用实例

扫描电镜下的多孔玻璃膜

   

3微米孔

 

   

0.5微米孔